The Asunafo South District is one of the 261 Metropolitan, Municipal and District Assemblies (MMDAs) in Ghana, and forms part of the 6 of Municipalities and Districts in the Ahafo Region

It was carved out from the then Asunafo District. The Asunafo South District was established by a Legislative Instrument L.I. 1773 in 2012.

The District has an estimated land size of about 3,737 kilometre square. The Asunafo South District is located at the southern part of the Ahafo Region with it's capital as Kukuom.

The District shares boarders with the Asunafo North Municipal to the north and the Juabuso District to the south-west. Other adjoining Districts are the Sefwi Wiaso Municipal in the Western North Region to the south-east, and Atwima Mponua District in the Ashanti Region to the east.

The population of the District according to 2021 population and housing census stands at 91,693 with 46,956 males and 44,737 females.